杂交瘤技术的发展为获得无限量的单克隆抗体（mAb）创造了可能，这些抗体对任何目标都具有高度的特异性和亲和力，并将mAb引入广泛的应用中；然而，mAb的体积庞大，生产成本高，工程繁琐，阻碍了它们在某些应用领域的合理发展。 在《单域抗体。方法和协议》中，专家们研究了单变域抗体片段，被称为VH、VL、VHH或VNAR。这些片段是最小的完整的抗原结合片段，可以以低成本重组生产。 以非常成功的Methods in Molecular Biology™系列格式编写，各章包括各自主题的介绍，必要的材料和试剂清单，逐步的、易于复制的实验室方案，以及关于故障排除和避免已知陷阱的提示。

The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications.  In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls