科研星球

「电子书」Cancer Cell Culture. Methods and Protocols 癌细胞培养. 方法和协议 PDF

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专家研究人员详细描述了他们在实验室培养癌细胞的最有成效和最新的方法。有一些方法可以描述和验证细胞系,分离和发展特定类型的癌症细胞,并开发新的细胞系模型。提供了评估克隆性、细胞增殖、细胞凋亡、粘附、迁移、侵袭、衰老、血管生成和细胞周期参数的功能检测方法。其他方法允许对癌细胞进行改造,以进行转染、抗药性开发、永生化和体内转移;不同类型细胞的共培养;以及检测和处理污染。


Expert researchers describe in detail their most productive and up-to-date methods for growing cancer cells in the laboratory. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cancer cells for transfection, development of drug resistance, immortalization, and transfer in vivo; the co-culture of different cell types; and the detection and treatment of contamination.

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