科研星球

「电子书」PCR Protocols (Methods in Molecular Biology) PCR 方法(分子生物学方法) PDF

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PCR技术以灵活性和稳健性著称,通过众多的发展,包括对具有一系列特性以适应特定应用的热稳定DNA聚合酶的鉴定,PCR技术在不断改进。PCR协议,第三版选择了最近开发的工具和技巧,由领域领先的作者贡献,因为他们增加了更普遍建立的方法的重要价值。除了最前沿的方法,本卷还介绍了许多核心应用,如PCR克隆和测序、表达、拷贝数或甲基化谱分析、"DNA指纹"、诊断、蛋白质工程、相互作用筛选,以及强调工作流程考虑因素和污染控制的章节,这些对所有PCR方法都至关重要。本书采用非常成功的Methods in Molecular Biology™系列格式编写,各章包括各自主题的介绍、必要的试剂和材料清单、逐步的、易于重复的实验室方案,以及关于故障排除和避免已知陷阱的提示。《PCR协议第三版》具有权威性和时效性,旨在进一步阐明这一重要技术,同时也为各种背景的科学家提供具有广泛应用的核心原则。


Known for flexibility and robustness, PCR techniques continue to improve through numerous developments, including the identification of thermostable DNA polymerases which exhibit a range of properties to suit given applications. PCR Protocols, Third Edition selects recently developed tools and tricks, contributed by field-leading authors, for the significant value that they add to more generally established methods. Along with the cutting-edge methodologies, this volume describes many core applications, such as PCR cloning and sequencing, expression, copy number or methylation profile analysis, ‘DNA fingerprinting’, diagnostics, protein engineering, interaction screening as well as a chapter highlighting workflow considerations and contamination control, crucial for all PCR methods. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary reagents and materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, PCR Protocols, Third Edition seeks to further elucidate this essential technique while also providing core principles with broad applications for scientists of all backgrounds.

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