本卷详细介绍了体外合成具有有利特性的mRNA的实验室方案，通过各种技术将其引入细胞，并对生理和临床后果进行测定，如蛋白质替代和癌症免疫疗法。描述了mRNA中的结构特征的合成技术，这些技术提供了研究工具，如荧光发射，点击化学，光化学交联，并且产生的mRNA在细胞中的稳定性增加，翻译效率提高，并减少先天免疫反应的激活。描述了临床应用的方案，如树突状细胞的大规模转染，GMP级mRNA的生产，重定向T细胞特异性，以及RNA疫苗的分子佐剂的使用。本书采用非常成功的分子生物学方法系列格式编写，各章包括各自主题的介绍、所需材料和试剂清单、分步实验方案，以及排除故障和避免已知陷阱的提示。

《合成mRNA：生产、引入细胞和生理后果》对实验室研究人员和对这一强大而迅速发展的技术感兴趣的临床医生来说，都是宝贵的前沿资源。

This volume presents detailed laboratory protocols for in vitro synthesis of mRNA with favorable properties, its introduction into cells by a variety of techniques, and the measurement of physiological and clinical consequences such as protein replacement and cancer immunotherapy. Synthetic techniques are described for structural features in mRNA that provide investigational tools such as fluorescence emission, click chemistry, photo-chemical crosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced activation of the innate immune response. Protocols are described for clinical applications such as large-scale transfection of dendritic cells, production of GMP-grade mRNA, redirecting T cell specificity, and use of molecular adjuvants for RNA vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resource for both laboratory investigators and clinicians interested in this powerful and rapidly evolving technology.