科研星球

细胞增殖/毒性检测方法比较及CCK-8法活力测定

今天小编再分享一下 CCK-8法的活力测定,顺便再整理了几种细胞增殖/毒性检测方法优势比较。

 

活力计算

*细胞活力:细胞增殖活力或细胞毒性活力

活力计算:细胞活力×(%)=[A(加药)-A(空白)]/[A0加药)-A(空白)×100

A(加药):具有细胞、CCK-8溶液和药物溶液的孔的吸光度

A(空白):具有培养基和CCK-8溶液而没有细胞的孔的吸光度

A0加药):具有细胞、CCK-8溶液而没有药物溶液的孔的吸光度


已发表文献数据

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文献引用

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CCK法与其他细胞增殖/毒性检测方法的优势比较

 

检测方法

MTT法

XTT法

WST-1法

CCK 法

甲臜产物的水溶性

差(需加有机溶剂溶解检测)

产品性状

粉末

2瓶溶液

溶液

1瓶溶液

使用方法

配成溶液后使用

现配现用

即开即用

即开即用

检测灵敏度

很高

很高

检测时间

较长

较短

较短

最短

检测波长

560-600nm

420-480nm

420-480nm

430-490nm

细胞毒性

高,细胞形态完全消失

很低,细胞形态不变

很低,细胞形态不变

很低,细胞形态不变

试剂稳定性

一般

较差

一般

很好

批量样品检测

可以

非常适合

非常适合

非常适合

便捷程度

一般

便捷

便捷

非常便捷



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