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「电子书」Practical Protein Chromatography PDF 实用蛋白质色谱法 英文原版

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Practical Protein Chromatography PDF 实用蛋白质色谱法 英文原版


Kenney and Fowell's exceptionally useful new book, Practical Protein Chromatography, brings all the forefront methods of protein separation and purification directly to your laboratory benchtop. The book treats not only powerful analytical separations, but also the small- and large-scale preparative techniques that are currently applicable throughout biological and biomedical research. Chapters, written by experts in each of the techniques covered, treat a variety of methods usable in virtually every laboratory with a chromatographic system. Topics include: immunoaffinity chromatography and selection of antibodies • lectin affinity and dye-ligand chromatography • exploiting weak affinities • alternative coupling chemistries • biospecific affinity elution • size-exclusion HPLC of proteins • chromatofocusing • determination of purity and yield • specialized chromatographic techniques. The established tradition of Methods in Molecular Biology is to present techniques that guarantee optimum results. Everyone doing protein chromatography today-at any level of proficiency-will find Practical Protein Chromatography an indispensable, hands-on guide to the effective use of these methodologies.


Kenney和Fowell的新书《实用蛋白质色谱法》异常实用,它将蛋白质分离和纯化的所有前沿方法直接带到了你的实验室台面上。本书不仅论述了功能强大的分析分离,还论述了目前适用于整个生物和生物医学研究的小型和大型制备技术。本书的各章由各技术领域的专家撰写,论述了几乎所有配备色谱系统的实验室都可以使用的各种方法。主题包括:免疫亲和色谱和抗体的选择--凝集素亲和和染料配体色谱--利用弱亲和力--替代性偶联化学--生物特异性亲和洗脱--蛋白质的大小排阻HPLC--色谱聚焦--纯度和产率的测定--专用色谱技术。分子生物学方法》的既定传统是提出保证最佳结果的技术。今天每一个从事蛋白质色谱的人--无论其熟练程度如何--都会发现《实用蛋白质色谱》是有效使用这些方法的不可缺少的实践指南。

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