这本详细的书介绍了一套对重组免疫球蛋白和替代支架工程、诱导剂开发和分子印迹聚合物（MIPs）生成领域的研究人员有用的协议。第一部分包括处理基于氨基酸的合成抗体的方法。详细介绍了关于抗体库生成的简要协议，以及抗体选择、表征和验证的技术。这一部分由一个生物信息学平台的简要描述完成，该平台在研究和开发过程中支持抗体工程。第二部分包含了关于选择和表征诱导剂分子的基本程序，第三部分描述了MIP生成和应用的基本过程。为非常成功的《分子生物学方法》系列编写，各章包括各自主题的介绍、必要的材料和试剂清单、逐步进行的、易于复制的实验室方案，以及关于故障排除和避免已知陷阱的提示。

具有权威性和实用性。《方法和协议》是科学家们寻求推动抗体研究的重要研究的理想指南。

This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic antibodies. Brief protocols about the generation of antibody libraries are detailed, as well as techniques for antibody selection, characterization, and validation. This section is completed by a brief description of a bioinformatics platform that supports antibody engineering during research and development. Part II contains basic procedures about the selection and characterization of aptamer molecules, and Part III describes fundamental processes of MIP generation and application. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Synthetic Antibodies: Methods and Protocols is an ideal guide for scientists seeking to propel the vital study of antibody research.