科研星球

「电子书」Histology Protocols 组织学方案 PDF

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我们对疾病发病机理的了解大多来自于对组织学材料的系统和仔细研究,组织学已被证明是一个非常有价值的研究领域。在《组织学方案》中,该领域的专家研究人员贡献了涉及组织制备的详细方法,提供了固定、包埋和处理的概述,以及从组织学切片中检索RNA所需的技术,随后是常规和专业组织学染色技术的部分,包括免疫、凝集素和杂交组织化学、组织学染色,以及原位识别缺氧和凋亡的具体方法,并详细介绍了成像和图像分析的进展。本书采用非常成功的Methods in Molecular Biology™系列格式编写,各章包括各自主题的介绍、必要的材料和试剂清单、逐步的、易于重复的实验方案,以及关于故障排除和避免已知陷阱的说明。


《组织学方案》具有权威性和前沿性,将为分子生物学家提供基本的组织化学技术,为组织学家提供发挥资源潜力所必需的分子技术。


With much of what we know about the pathogenesis of disease coming from the systematic and careful study of histological material, histology has proven to be an incredibly valuable area of study. In Histology Protocols, expert researchers in the field contribute detailed methods involving the preparation of tissue, providing an overview of fixation, embedding, and processing, along with the required techniques for the retrieval of RNA from histological sections, followed by sections on routine and specialist histological staining techniques, including immuno-, lectin, and hybridization histochemistry, histological staining, as well as specific methods for the in situ identification of hypoxia and apoptosis, and details on the advances in imaging and image analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Histology Protocols will provide molecular biologists with the basic histochemical techniques and histologists with the molecular techniques necessary to realize the potential of their resource.


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