本书第二版探讨了目前用于元基因组学的各种基于功能的技术的最新工具。本书的各章讨论了这些技术所涉及的所有工作步骤，如。从土壤和海洋样品中分离DNA，然后构建和筛选各种酶和生物分子的文库；目前用于从陆地和海洋生境中分离DNA和构建大、小插入文库的方法；在非大肠杆菌宿主中建立元基因组文库的方法；用于元基因组DNA功能驱动挖掘的新分子工具；以及与生物技术和生态学相关的各种不同基因编码酶的筛选方案。元基因组学。第二版还提供了磷酸酶、多羟基烷酸、代谢相关酶、立体选择水解酶和微生物信号的详细筛选协议，以发现次级代谢物。本书采用非常成功的分子生物学方法系列格式编写，各章包括各自主题的介绍、必要的材料和试剂清单、逐步的、易于重复的实验方案，以及排除故障和避免已知陷阱的提示。

全面而前沿的《元基因组学：方法和协议》，第二版是一个最新的元基因组协议和工具的集合，用于回收许多主要类型的生物催化剂，并允许研究人员在任何微生物学实验室中轻松设置这些屏幕。

This second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by the construction and screening of libraries for diverse enzymes and biomolecules; current methods used to isolate DNA and construct large- and small-insert libraries from terrestrial and marine habitats; methods for establishing metagenome libraries in none-E.coli hosts; new molecular tools used for function-driven mining of metagenomic DNA; and screening protocols for a wide array of different genes encoding enzymes with relevance to biotechnology and ecology. Metagenomics: Methods and Protocols, Second Edition also provides detailed screening protocols for phosphatases, poly-hydroxyalkanoate, metabolism-related enzymes, stereoselective hydrolases, and microbial signals for the discovery of secondary metabolites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Metagenomics: Methods and Protocols, Second Edition is a collection of up-to-date metagenome protocols and tools for the recovery of numerous major types of biocatalysts, and allows researchers to easily setup these screens in any microbiology laboratory.