本书详细介绍了一系列最新的方法，包括在不同的生物学背景下识别新型ncRNAs及其靶标、功能注释和疾病关联。各章引导读者了解疾病特异性ncRNA的概述、ncRNA发现的计算方法和工作流程、基于高通量测序数据的注释、ncRNA分析的生物信息学工具和数据库、基于网络的方法以及ncRNA介导的基因调控的动力学模型。本书采用非常成功的分子生物学方法系列格式编写，各章包括各自主题的介绍、必要的材料和试剂清单、逐步的、易于重复的实验方案，以及排除故障和避免已知陷阱的提示。

权威而前沿的《非编码RNA的计算生物学。方法和协议》旨在提供最先进的计算方法和方法的集合，这对于对ncRNA领域感兴趣的研究人员来说是有价值的。

This volume details a collection of state-of-art methods including identification of novel ncRNAs and their targets, functional annotation and disease association in different biological contexts. Chapters guide readers through an overview of disease-specific ncRNAs, computational methods and workflows for ncRNA discovery, annotation based on high-throughput sequencing data, bioinformatics tools and databases for ncRNA analyses, network-based methods, and kinetic modelling of ncRNA-mediated gene regulation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Computational Biology of Non-Coding RNA: Methods and Protocols aims to provide a state-of-the-art collection of computational methods and approaches that will be of value to researchers interested in ncRNA field.