本书探讨了RNAi和CRISPR干扰作为抑制基因表达的一般方法的用途，重点介绍了它们的生物学功能、设计、化学修饰、递送和临床前/临床应用。除了相关背景外，本书各章还讨论了处理RNAi和CRISPR应用最新进展的简单而准确的协议，并探讨了这些方法之间的差异。本书以非常成功的《分子生物学方法》系列格式编写，各章包括对各自主题的介绍、必要的材料和试剂清单、逐步的、易于重复的实验室方案，以及故障排除和避免已知陷阱的提示。全面而前沿的RNA干扰和CRISPR技术。技术进展和新的治疗机会》是任何对该领域感兴趣的科学家、教师、研究生、博士后和临床医生的宝贵资源。本书还有利于任何在生物技术和制药公司从事研发工作的人，他们想更多地了解这些技术，以及它们在生物学和医学中的应用。

This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine.