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「电子书」Quantitative Real-Time PCR: Methods and Protocols 实时荧光定量 PCR 方法及其实验研究 PDF

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《定量实时PCR。方法和协议》侧重于qPCR的不同应用,从微生物检测(包括病毒和细菌)到病理应用。有几章涉及质量问题,涉及起始材料的质量、执行检测和设定实验计划所需的最低信息的知识,而其他章节则侧重于转化医学应用,按照其医学应用的近似逻辑顺序排列。本书最后一部分让你了解一种新兴的数字PCR技术,它是一种独特的qPCR方法,用于测量核酸,特别适合于低水平的检测和发展无创诊断。本书为《分子生物学方法》丛书而写,大部分章节都包括各自主题的介绍、必要材料和试剂清单、步骤、实验方案以及故障排除和避免已知陷阱的提示。


《定量实时PCR: 方法和协议》旨在帮助研究人员设计与其研究领域相关的基于qPCR的新方法。


Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols and tips on troubleshooting and avoiding known pitfalls.


Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation.


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