科研星球

「电子书」Single Cell Methods: Sequencing and Proteomics 单细胞测序与蛋白质组学 PDF

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本书为研究单个细胞水平的生物学提供了全面的概述。各章分为八个部分,详细介绍了单细胞实验室、单细胞DNA-seq、RNA-seq、单细胞蛋白质组学和表观遗传学、单细胞多组学、单细胞筛选和单细胞活体成像。本书采用非常成功的《分子生物学方法》系列格式编写,各章包括各自主题的介绍、必要的材料和试剂清单、逐步的、易于重复的实验方案,以及排除故障和避免已知陷阱的提示。


This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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