A collection of readily reproducible techniques for the study of mRNA processing and metabolism. These cutting-edge techniques range from cotranscriptional processing events that occur while the mNA is engaged with elongating RNA polymerase II, to in vivo and in vitro splicing and its biochemical analysis, and alternative splicing. Additional methods cover mRNA export, the recovery and analysis of mRNP complexes, cytoplasmic translation, mRNA degradation in vivo and in vitro, and the controversial concept of nuclear translation. Each proven protocol is described in step-by-step detail and contains a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding know pitfalls.

用于研究mRNA加工和代谢的一系列可重复的技术。这些前沿技术的范围从mRNA与RNA聚合酶II伸长时发生的共转录处理事件，到体内和体外剪接及其生化分析，以及替代性剪接。其他方法包括mRNA的导出，mRNP复合物的回收和分析，细胞质翻译，体内和体外的mRNA降解，以及有争议的核翻译概念。每一个成熟的协议都有详细的步骤描述，并包含一个背景介绍，概述技术背后的原理，设备和试剂清单，以及故障排除和避免已知陷阱的提示。